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Radiation Induces Prompt Live-Cell Metabolic Fluxes: an In-Vitro Demonstration

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자료유형	E-Book
개인저자	Campos, David Daniel.
단체저자명	The University of Wisconsin - Madison. Medical Physics.
서명/저자사항	Radiation Induces Prompt Live-Cell Metabolic Fluxes: an In-Vitro Demonstration.
발행사항	[S.l.] : The University of Wisconsin - Madison., 2017
발행사항	Ann Arbor : ProQuest Dissertations & Theses, 2017
형태사항	130 p.
소장본 주기	School code: 0262.
ISBN	9780438159549
일반주기	Source: Dissertation Abstracts International, Volume: 79-11(E), Section: B. Adviser: Michael W. Kissick.
요약	Radiation therapy is a very important component of cancer treatments. Considerable advancements in radiotherapy technology have allowed for more uniform dose distributions, as well as improved the ability to avoid organs at risk. These important
요약	For this study, metabolic fluxes in two cell lines, a cancerous urothelial carcinoma cell line (T24) and a comparable normal cell line, Normal Oral Keratinocytes (NOK) were assessed by fluorescence lifetime imaging microscopy (FLIM) of the reduc
요약	To link changes in metabolism with the local tumor microenvironment, we also assessed expression of transcription factor subunit HIF-1alpha via immunofluorescence staining for both cell lines. The HIF-1 complex serves as a transcription factor f
요약	Because cellular mitochondria play a major role in cellular metabolism through oxidative phosphorylation (oxphos) and oxphos-generated reactive oxygen species (ROS), we quantified cellular ROS and mitochondrial membrane polarization (MMP) in eac
요약	Because of the potential link between HIF-1, radioprotection and metabolism, expression of Hexokinase II (HK-II), a transcription target of HIF-1, was quantified using the Western Blot gel electrophoresis technique. Hexokinase is a unique isofo
요약	Together, these data indicate that following irradiation T24 cells have a capacity to increase levels of glycolysis as compared to NOK cells. We hypothesize that this capability may be driven by T24 cells' expression of HIF-1 to transcribe HK-II
일반주제명	Oncology. Biomedical engineering. Biology.
언어	영어
기본자료 저록	Dissertation Abstracts International79-11B(E). Dissertation Abstract International
대출바로가기	http://www.riss.kr/pdu/ddodLink.do?id=T14996601