| LDR | | 02938nmm uu200481 4500 |
| 001 | | 000000334606 |
| 005 | | 20240805180639 |
| 008 | | 181129s2017 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438159549 |
| 035 | |
▼a (MiAaPQ)AAI10289623 |
| 035 | |
▼a (MiAaPQ)wisc:14552 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 082 | 0 |
▼a 616.99 |
| 100 | 1 |
▼a Campos, David Daniel. |
| 245 | 10 |
▼a Radiation Induces Prompt Live-Cell Metabolic Fluxes: an In-Vitro Demonstration. |
| 260 | |
▼a [S.l.] :
▼b The University of Wisconsin - Madison.,
▼c 2017 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2017 |
| 300 | |
▼a 130 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-11(E), Section: B. |
| 500 | |
▼a Adviser: Michael W. Kissick. |
| 502 | 1 |
▼a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2017. |
| 520 | |
▼a Radiation therapy is a very important component of cancer treatments. Considerable advancements in radiotherapy technology have allowed for more uniform dose distributions, as well as improved the ability to avoid organs at risk. These important |
| 520 | |
▼a For this study, metabolic fluxes in two cell lines, a cancerous urothelial carcinoma cell line (T24) and a comparable normal cell line, Normal Oral Keratinocytes (NOK) were assessed by fluorescence lifetime imaging microscopy (FLIM) of the reduc |
| 520 | |
▼a To link changes in metabolism with the local tumor microenvironment, we also assessed expression of transcription factor subunit HIF-1alpha via immunofluorescence staining for both cell lines. The HIF-1 complex serves as a transcription factor f |
| 520 | |
▼a Because cellular mitochondria play a major role in cellular metabolism through oxidative phosphorylation (oxphos) and oxphos-generated reactive oxygen species (ROS), we quantified cellular ROS and mitochondrial membrane polarization (MMP) in eac |
| 520 | |
▼a Because of the potential link between HIF-1, radioprotection and metabolism, expression of Hexokinase II (HK-II), a transcription target of HIF-1, was quantified using the Western Blot gel electrophoresis technique. Hexokinase is a unique isofo |
| 520 | |
▼a Together, these data indicate that following irradiation T24 cells have a capacity to increase levels of glycolysis as compared to NOK cells. We hypothesize that this capability may be driven by T24 cells' expression of HIF-1 to transcribe HK-II |
| 590 | |
▼a School code: 0262. |
| 650 | 4 |
▼a Oncology. |
| 650 | 4 |
▼a Biomedical engineering. |
| 650 | 4 |
▼a Biology. |
| 690 | |
▼a 0992 |
| 690 | |
▼a 0541 |
| 690 | |
▼a 0306 |
| 710 | 20 |
▼a The University of Wisconsin - Madison.
▼b Medical Physics. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-11B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0262 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2017 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T14996601
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자 |