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Applying Protein-Peptide Interactions to Fluorescently Label Proteins in S. Cerevisiae
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| 자료유형 | E-Book |
|---|---|
| 개인저자 | Hinrichsen, Michael. |
| 단체저자명 | Yale University. |
| 서명/저자사항 | Applying Protein-Peptide Interactions to Fluorescently Label Proteins in S. Cerevisiae. |
| 발행사항 | [S.l.] : Yale University., 2018 |
| 발행사항 | Ann Arbor : ProQuest Dissertations & Theses, 2018 |
| 형태사항 | 121 p. |
| 소장본 주기 | School code: 0265. |
| ISBN | 9780438193673 |
| 일반주기 |
Source: Dissertation Abstracts International, Volume: 79-11(E), Section: B.
Adviser: Lynne Regan. |
| 요약 | Cells are highly complex, organized and dynamic machines, driven by intricate protein-based machinery. Understanding how this protein machinery produces the cellular processes we observe requires more than simply characterizing the individual pa |
| 요약 | While a number of methods can be used to fluorescently label a protein of interest, there is no one method that is suitable for all imaging applications. Oftentimes, picking the appropriate labeling method requires balancing a variety of competi |
| 요약 | SpyCatcher/SpyTag are a protein/peptide pair that bind one another and form an irreversible isopeptide bond in solution. I harnessed this interaction pair to fluorescently label proteins in vivo by fusing SpyTag (13 amino acids) to the protein o |
| 요약 | Additionally. I have also investigated the use of a reversible protein/peptide interaction to fluorescently label proteins in S. cerevisiae. TRAPs are artificial binding domains developed in the Regan lab that bind 5 amino acid long, C-terminal |
| 요약 | Thus, I have shown that a variety of protein/peptide interactions can be used to fluorescently label proteins in vivo and have developed the tools and protocols necessary to fluorescently label proteins of interest in yeast. Our SpyCatcher/SpyTa |
| 일반주제명 | Biochemistry. Biophysics. Cellular biology. |
| 언어 | 영어 |
| 기본자료 저록 | Dissertation Abstracts International79-11B(E). Dissertation Abstract International |
| 대출바로가기 | http://www.riss.kr/pdu/ddodLink.do?id=T15000822 |
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| No. | 등록번호 | 청구기호 | 소장처 | 도서상태 | 반납예정일 | 예약 | 서비스 | 매체정보 |
|---|---|---|---|---|---|---|---|---|
| 1 | WE00025730 | 574 | 가야대학교// | 대출가능 |
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