| LDR | | 02555nmm uu200457 4500 |
| 001 | | 000000331403 |
| 005 | | 20240805164320 |
| 008 | | 181129s2018 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438193673 |
| 035 | |
▼a (MiAaPQ)AAI10927797 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 082 | 0 |
▼a 574 |
| 100 | 1 |
▼a Hinrichsen, Michael. |
| 245 | 10 |
▼a Applying Protein-Peptide Interactions to Fluorescently Label Proteins in S. Cerevisiae. |
| 260 | |
▼a [S.l.] :
▼b Yale University.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 121 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-11(E), Section: B. |
| 500 | |
▼a Adviser: Lynne Regan. |
| 502 | 1 |
▼a Thesis (Ph.D.)--Yale University, 2018. |
| 520 | |
▼a Cells are highly complex, organized and dynamic machines, driven by intricate protein-based machinery. Understanding how this protein machinery produces the cellular processes we observe requires more than simply characterizing the individual pa |
| 520 | |
▼a While a number of methods can be used to fluorescently label a protein of interest, there is no one method that is suitable for all imaging applications. Oftentimes, picking the appropriate labeling method requires balancing a variety of competi |
| 520 | |
▼a SpyCatcher/SpyTag are a protein/peptide pair that bind one another and form an irreversible isopeptide bond in solution. I harnessed this interaction pair to fluorescently label proteins in vivo by fusing SpyTag (13 amino acids) to the protein o |
| 520 | |
▼a Additionally. I have also investigated the use of a reversible protein/peptide interaction to fluorescently label proteins in S. cerevisiae. TRAPs are artificial binding domains developed in the Regan lab that bind 5 amino acid long, C-terminal |
| 520 | |
▼a Thus, I have shown that a variety of protein/peptide interactions can be used to fluorescently label proteins in vivo and have developed the tools and protocols necessary to fluorescently label proteins of interest in yeast. Our SpyCatcher/SpyTa |
| 590 | |
▼a School code: 0265. |
| 650 | 4 |
▼a Biochemistry. |
| 650 | 4 |
▼a Biophysics. |
| 650 | 4 |
▼a Cellular biology. |
| 690 | |
▼a 0487 |
| 690 | |
▼a 0786 |
| 690 | |
▼a 0379 |
| 710 | 20 |
▼a Yale University. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-11B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0265 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15000822
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자 |