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Structural Characterization of Ribonucleic Acids and Their Complexes by Negative-ion Mode Mass Spectrometry

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자료유형	E-Book
개인저자	Ileka, Kevin M.
단체저자명	University of Michigan. Chemistry.
서명/저자사항	Structural Characterization of Ribonucleic Acids and Their Complexes by Negative-ion Mode Mass Spectrometry.
발행사항	[S.l.] : University of Michigan., 2018
발행사항	Ann Arbor : ProQuest Dissertations & Theses, 2018
형태사항	146 p.
소장본 주기	School code: 0127.
ISBN	9780438126572
일반주기	Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B. Adviser: Kristina I. Hakansson.
요약	Ribonucleic acids (RNAs) form complexes with deoxyribonucleic acids, proteins, other RNAs, and smaller ligands. Detailed knowledge of RNA interaction sites provides a basis for understanding functions. With limited analytical techniques availabl
요약	Negative-ion electron capture dissociation (niECD) involves &sim
요약	Electrospray ionization (ESI) of a model RNA hairpin from native-like (10 mM ammonium acetate) and methanol-containing (up to 50%) solvents resulted in identical charge state distributions, suggesting a minor methanol effect on overall conformat
요약	Crosslinking techniques coupled with MS provide an alternative tool for identifying RNA interaction sites. We show that collisional activation can provide full sequence coverage of the RNA moiety within non-covalent RNA-peptide complexes
요약	Microfluidics is a highly efficient technology for biological analysis. Microfluidic-type approaches, including nano-ESI and nano-LC, coupled with MS provide several advantages, e.g., limited sample consumption and enhanced sensitivity. In order
요약	Overall, this dissertation describes the utility of MS (and its associated tools) for the study of RNA, RNA-small molecule, and RNA-protein complexes.
일반주제명	Molecular chemistry.
언어	영어
기본자료 저록	Dissertation Abstracts International79-12B(E). Dissertation Abstract International
대출바로가기	http://www.riss.kr/pdu/ddodLink.do?id=T15000549