| LDR | | 00000nmm u2200205 4500 |
| 001 | | 000000330863 |
| 005 | | 20241105134156 |
| 008 | | 181129s2018 ||| | | | eng d |
| 020 | |
▼a 9780438126572 |
| 035 | |
▼a (MiAaPQ)AAI10903051 |
| 035 | |
▼a (MiAaPQ)umichrackham:001182 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 049 | 1 |
▼f DP |
| 082 | 0 |
▼a 540 |
| 100 | 1 |
▼a Ileka, Kevin M. |
| 245 | 10 |
▼a Structural Characterization of Ribonucleic Acids and Their Complexes by Negative-ion Mode Mass Spectrometry. |
| 260 | |
▼a [S.l.] :
▼b University of Michigan.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 146 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B. |
| 500 | |
▼a Adviser: Kristina I. Hakansson. |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of Michigan, 2018. |
| 520 | |
▼a Ribonucleic acids (RNAs) form complexes with deoxyribonucleic acids, proteins, other RNAs, and smaller ligands. Detailed knowledge of RNA interaction sites provides a basis for understanding functions. With limited analytical techniques availabl |
| 520 | |
▼a Negative-ion electron capture dissociation (niECD) involves &sim |
| 520 | |
▼a Electrospray ionization (ESI) of a model RNA hairpin from native-like (10 mM ammonium acetate) and methanol-containing (up to 50%) solvents resulted in identical charge state distributions, suggesting a minor methanol effect on overall conformat |
| 520 | |
▼a Crosslinking techniques coupled with MS provide an alternative tool for identifying RNA interaction sites. We show that collisional activation can provide full sequence coverage of the RNA moiety within non-covalent RNA-peptide complexes |
| 520 | |
▼a Microfluidics is a highly efficient technology for biological analysis. Microfluidic-type approaches, including nano-ESI and nano-LC, coupled with MS provide several advantages, e.g., limited sample consumption and enhanced sensitivity. In order |
| 520 | |
▼a Overall, this dissertation describes the utility of MS (and its associated tools) for the study of RNA, RNA-small molecule, and RNA-protein complexes. |
| 590 | |
▼a School code: 0127. |
| 650 | 4 |
▼a Molecular chemistry. |
| 690 | |
▼a 0431 |
| 710 | 20 |
▼a University of Michigan.
▼b Chemistry. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-12B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0127 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15000549
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자
▼b 관리자 |