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Identification of Important Cell Cycle Regulators and Novel Genes in Specific Tissues Using Microarray Analysis, Bioinformatics and Molecular Tools

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자료유형	E-Book
개인저자	Zhang, Jibin.
단체저자명	The Ohio State University. Animal Sciences.
서명/저자사항	Identification of Important Cell Cycle Regulators and Novel Genes in Specific Tissues Using Microarray Analysis, Bioinformatics and Molecular Tools.
발행사항	[S.l.] : The Ohio State University., 2015
발행사항	Ann Arbor : ProQuest Dissertations & Theses, 2015
형태사항	192 p.
소장본 주기	School code: 0168.
ISBN	9780438097421
일반주기	Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B. Adviser: Michael Davis.
요약	Study of cell cycle activity is critical to understand development of adipose tissue, because the switch on and off of cell cycle activity is tightly related to hyperplasia and hypotrophy of the fat cells. Three cell cycle inhibitors---CCNG2, CD
요약	The three important cell cycle inhibitors showed higher expression in fat cells than in preadipocytes and increased exprerssion during primary cell culture and development of adipose tissue in quantitative real-time PCR (qPCR) or western blottin
요약	In broiler chickens, expression of CCNG2, CDKN2C, CCND3 and ANAPC5 reaches a peak at post-hatch day 5 and decreases in the later stages, suggesting that day 5 may be an important time with active proliferation and differentiation of preadipocyte
요약	In the third experiment, some tissue specific secretory factors in mouse were selected based on GEO data analysis and bioinformatic tools. The tissue specific expressions of the selected genes were confirmed using semi-quantitative PCR. Secretio
요약	In the fourth study, a novel adipose specific gene HPS4 was revealed by DNA microarray analysis. Its higher expression in adipose tissue than in other tissues and in fat cells than in stromal vascular cells was detected by qPCR or western blotti
요약	In general, our studies not only developed a strategy to identify genes with important effects and novel genes in adipose and other tissues, but also increased our understanding about adipose tissue and provided some guidance to studies in other
일반주제명	Zoology. Bioinformatics. Animal sciences. Biology.
언어	영어
기본자료 저록	Dissertation Abstracts International79-10B(E). Dissertation Abstract International
대출바로가기	http://www.riss.kr/pdu/ddodLink.do?id=T15000273