| LDR | | 02350nmm uu200409 4500 |
| 001 | | 000000334586 |
| 005 | | 20240805180614 |
| 008 | | 181129s2017 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438024175 |
| 035 | |
▼a (MiAaPQ)AAI10280701 |
| 035 | |
▼a (MiAaPQ)wisc:14376 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 082 | 0 |
▼a 574 |
| 100 | 1 |
▼a Carrocci, Tucker Joe. |
| 245 | 10 |
▼a Biochemical and Genetic Characterization of Factors that Influence Branchsite Selection during Pre-mRNA Splicing in Yeast. |
| 260 | |
▼a [S.l.] :
▼b The University of Wisconsin - Madison.,
▼c 2017 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2017 |
| 300 | |
▼a 268 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B. |
| 500 | |
▼a Adviser: Aaron A. Hoskins. |
| 502 | 1 |
▼a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2017. |
| 520 | |
▼a RNA splicing plays many critical roles in eukaryotic gene expression, yet even 40 years after its discovery, the mechanism by which the spliceosome recognizes and removes introns is still poorly understood. In my thesis, I describe methods to pr |
| 520 | |
▼a In my thesis, I discuss methods to label RNA fluorescently. I used MS2 fusion proteins tags to the label RNAs in living yeast using small molecule fluorophores. These tags allow for visualization of localized RNAs and demonstrate that organic fl |
| 520 | |
▼a In the second portion of my thesis, I discuss work on the role of splicing factors that contribute to branchsite choice. I explored the role of cancer causing mutations in a protein called SF3b1 using the yeast spliceosome. Mutations of SF3b1 re |
| 520 | |
▼a Finally, I used smFRET to observe dynamics in the splicing factor Prp5, which ensures proper branchsite usage by the spliceosome. I showed that Prp5 primarily adopts an open state in the absence of ATP and RNA but transitions to a closed state u |
| 590 | |
▼a School code: 0262. |
| 650 | 4 |
▼a Biochemistry. |
| 690 | |
▼a 0487 |
| 710 | 20 |
▼a The University of Wisconsin - Madison.
▼b Biochemistry-ALS. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-10B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0262 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2017 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T14996579
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자 |