| LDR | | 02417nmm uu200445 4500 |
| 001 | | 000000334348 |
| 005 | | 20240805180126 |
| 008 | | 181129s2015 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438284494 |
| 035 | |
▼a (MiAaPQ)AAI10969986 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 082 | 0 |
▼a 610 |
| 100 | 1 |
▼a Vu, Scott Khoi. |
| 245 | 12 |
▼a A New Biophysical Model for Translation Elongation to Predict and Optimize Heterologous Protein Production in Escherichia coli. |
| 260 | |
▼a [S.l.] :
▼b North Carolina State University.,
▼c 2015 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2015 |
| 300 | |
▼a 281 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B. |
| 500 | |
▼a Advisers: Donald L. Bitzer |
| 502 | 1 |
▼a Thesis (Ph.D.)--North Carolina State University, 2015. |
| 520 | |
▼a Translation is a biological process in which an organism produces specific polypeptides that fold into functional proteins. Heterologous protein production uses translation to synthesize proteins that are not normally expressed in production org |
| 520 | |
▼a A new computational-based biophysical model for translation elongation has been developed that provides: a) new insights into the mechanisms behind translation, e.g. elucidating programmed frameshifts prfB and dnaX, and b) a new approach to gene |
| 520 | |
▼a The model's predictive and optimization accuracy were verified by optimizing and expressing five model genes: gst (glutathione-S-transferase), pf0132 (alpha-glucosidase), clju_c11880 (alcohol dehydrogenase), bif1 (beta-galactosidase), and rt8_0 |
| 520 | |
▼a Based on the results presented in this dissertation and the literature published, the author contends that the determinants of protein yield from translation elongation are not just codon bias, but, rather, the convolution between aminoacyl-tRNA |
| 590 | |
▼a School code: 0155. |
| 650 | 4 |
▼a Biomedical engineering. |
| 650 | 4 |
▼a Biophysics. |
| 650 | 4 |
▼a Molecular biology. |
| 690 | |
▼a 0541 |
| 690 | |
▼a 0786 |
| 690 | |
▼a 0307 |
| 710 | 20 |
▼a North Carolina State University.
▼b Biomedical Engineering. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-12B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0155 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2015 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15001307
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자 |