| LDR | | 02517nmm uu200421 4500 |
| 001 | | 000000333927 |
| 005 | | 20240805174812 |
| 008 | | 181129s2018 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438125728 |
| 035 | |
▼a (MiAaPQ)AAI10902966 |
| 035 | |
▼a (MiAaPQ)umichrackham:001224 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 082 | 0 |
▼a 546 |
| 100 | 1 |
▼a Wallace, Rachel. |
| 245 | 10 |
▼a Folate Binding Protein as a Therapeutic Natural Nanotechnology. |
| 260 | |
▼a [S.l.] :
▼b University of Michigan.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 183 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B. |
| 500 | |
▼a Advisers: Mark M. Banaszak Holl |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of Michigan, 2018. |
| 520 | |
▼a Serum proteins interact with small molecules and nanoparticles in blood, resulting in protein coronas. Protein coronas influence the bioidentity of the molecules and nanoparticles, playing critical roles in biotransport, uptake, and fate. Target |
| 520 | |
▼a The research presented here is focused on characterizing and taking advantage of a particular protein corona -- the self-aggregation of serum folate binding protein (FBP). FBP is derived from cellular folate receptors, and both bind strongly to |
| 520 | |
▼a Using atomic force microscopy (AFM), we characterized the self-aggregation of FBP on a particle-by-particle basis at physiological concentrations. FBP self-aggregates into nanoparticles (forming FBPNP) at blood serum concentrations. We further e |
| 520 | |
▼a We studied FBP aggregation with two FA-poly(amidoamine) (PAMAM) dendrimers and two FA-poly(ethylene glycol) (PEG) conjugates. Fluorescence spectroscopy experiments showed that FA and FA-conjugates induced conformational changes throughout the pr |
| 520 | |
▼a Given the challenges associated with conjugated therapies, we sought to leverage FBP itself as a targeted vector. We hypothesized that pre-binding FBP to MTX would decrease toxicity and increase therapeutic efficacy. We tested this hypothesis in |
| 590 | |
▼a School code: 0127. |
| 650 | 4 |
▼a Inorganic chemistry. |
| 690 | |
▼a 0488 |
| 710 | 20 |
▼a University of Michigan.
▼b Chemistry. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-12B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0127 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15000474
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자 |