| LDR | | 02602nmm uu200445 4500 |
| 001 | | 000000333403 |
| 005 | | 20240805172949 |
| 008 | | 181129s2018 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438116894 |
| 035 | |
▼a (MiAaPQ)AAI10816999 |
| 035 | |
▼a (MiAaPQ)northwestern:14147 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 082 | 0 |
▼a 574 |
| 100 | 1 |
▼a Putzbach, William.
▼0 (orcid)0000-0002-2669-6654 |
| 245 | 10 |
▼a Toxicity Mediated by Seed-Dependent Off-Target Effects in RNA Interference. |
| 260 | |
▼a [S.l.] :
▼b Northwestern University.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 261 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-11(E), Section: B. |
| 500 | |
▼a Adviser: Marcus E. Peter. |
| 502 | 1 |
▼a Thesis (Ph.D.)--Northwestern University, 2018. |
| 520 | |
▼a Post-transcriptional gene silencing (PTGS) and, more specifically, RNA interference (RNAi) include the processes by which a small double-stranded RNA, 19 to 22 nucleotides (nts) long, negatively regulates the expression and/or translatability of |
| 520 | |
▼a Non-overlapping siRNAs that specifically and potently target the same gene would be predicted to evoke the same phenotypic change, provided the intended target performs an overt function. However, non-overlapping siRNAs with different seed seque |
| 520 | |
▼a Indeed, over 80% of commercially available siRNAs and short hairpin (sh)RNAs derived from CD95/CD95L mRNA sequences are toxic to cancer cells in the absence of the intended target site in these two genes, demonstrating this toxicity is independe |
| 520 | |
▼a These nonredundant CD95/CD95L-derived si/shRNAs all induce a novel form of cancer cell death, with distinct biochemical and morphological features best described as simultaneous activation of multiple death pathways. Interestingly, these toxic s |
| 520 | |
▼a The abundance of toxic shRNA sequences derived from the CD95L ORF, as identified in the screen, suggested the full-length mRNA may exhibit functionality independent of its role in translating for CD95L protein. Indeed, expression of wild type (W |
| 590 | |
▼a School code: 0163. |
| 650 | 4 |
▼a Cellular biology. |
| 650 | 4 |
▼a Biology. |
| 690 | |
▼a 0379 |
| 690 | |
▼a 0306 |
| 710 | 20 |
▼a Northwestern University.
▼b Life Sciences. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-11B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0163 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T14998311
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자 |