| LDR | | 03938nmm uu200481 4500 |
| 001 | | 000000333397 |
| 005 | | 20240805172942 |
| 008 | | 181129s2018 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438324855 |
| 035 | |
▼a (MiAaPQ)AAI10816955 |
| 035 | |
▼a (MiAaPQ)berkeley:17886 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 082 | 0 |
▼a 581 |
| 100 | 1 |
▼a Scheibel, Katherine E. |
| 245 | 10 |
▼a Identification and Characterization of Effector Proteins and Genomic Analysis of the Cucurbit Powdery Mildew Golovinomyces cichoracearum . |
| 260 | |
▼a [S.l.] :
▼b University of California, Berkeley.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 315 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 80-01(E), Section: B. |
| 500 | |
▼a Adviser: Shauna C. Somerville. |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of California, Berkeley, 2018. |
| 520 | |
▼a Powdery mildew, a fungal disease of plants, is one of the most significant causes of crop disease and yield loss worldwide. Collectively, this group of pathogens infects a diverse set of plant hosts, including wheat, barley, grape, and ornamenta |
| 520 | |
▼a When exposed to a susceptible host, G. cichoracearum forms a feeding structure within the plant cell, known as a haustorium. The fungus initiates changes in plant cell structure, gene expression, and nutrient transport to allow for its survival |
| 520 | |
▼a Here, we describe the development of an Agrobacterium tumefaciens -mediated tool for transiently silencing G. cichoracearum genes during infection of Arabidopsis. We demonstrate that this technique can be successfully employed at the early stag |
| 520 | |
▼a We then describe efforts to characterize these effectors in terms of gene expression, sub-cellular localization, the identification of plant interacting partners, bioinformatic prediction, and their roles in the suppression of the plant immune r |
| 520 | |
▼a GcEC10 is characterized as an RNase-like protein and is localized in the plant cytosol and nucleus. GcEC10 may interact with Arabidopsis proteins AtEDR4 and AtPHOS32, two proteins implicated in the plant immune response to pathogens. We determin |
| 520 | |
▼a GcEC8 shares no sequence or domain homology with any proteins outside of the powdery mildews, and may interact with the plant protein AXR3, an auxin-responsive transcription factor. We propose that GcEC8 may interfere with the plant auxin respon |
| 520 | |
▼a GcEC17 is a highly-conserved powdery mildew effector, and is also characterized as an RNase-like protein. GcEc17 is localized in the plant nucleus and cytosol. Two hypothetical Arabidopsis genes, At4g29905 and At3g32930, were predicted to intera |
| 520 | |
▼a We further describe efforts towards transient plasmid transformation of G. cichoracearum, which we hope will eventually lead to the ability of researchers to introduce the Cas9 genome editing system into the fungus. This might allow for the crea |
| 520 | |
▼a Finally, we describe a method developed to achieve the purification of high molecular weight genomic DNA from G. cichoracearum suitable for Pacific Biosciences Single Molecule Long-Read Sequencing, and the analysis of the genome sequence obtaine |
| 520 | |
▼a These experiments provide some of the first insights into the genome and effector biology of the enigmatic plant pathogen G. cichoracearum . By combining novel molecular techniques with next-generation sequencing approaches, we now have a more c |
| 590 | |
▼a School code: 0028. |
| 650 | 4 |
▼a Plant pathology. |
| 690 | |
▼a 0480 |
| 710 | 20 |
▼a University of California, Berkeley.
▼b Plant Biology. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 80-01B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0028 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T14998305
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자 |