| LDR | | 02469nmm uu200457 4500 |
| 001 | | 000000333266 |
| 005 | | 20240805172716 |
| 008 | | 181129s2018 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438173859 |
| 035 | |
▼a (MiAaPQ)AAI10815461 |
| 035 | |
▼a (MiAaPQ)washington:18454 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 082 | 0 |
▼a 574 |
| 100 | 1 |
▼a Lynch, Kelsey L. |
| 245 | 14 |
▼a The Limiting DNA Replication Initiation Factors Sld2 and Sld3 Influence Replication Origin Efficiency Independent of Time of Origin Firing in Saccharomyces cerevisiae. |
| 260 | |
▼a [S.l.] :
▼b University of Washington.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 150 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B. |
| 500 | |
▼a Adviser: Bonita J. Brewer. |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of Washington, 2018. |
| 520 | |
▼a During S-phase in Saccharomyces cerevisiae, DNA replication is initiated from roughly 300 chromosomal origins that are regulated both by DNA sequence and by interacting protein factors. The limited abundance of a set of trans-acting initiation p |
| 520 | |
▼a I found that depleting cells of either initiation factor slows growth rate, increases S-phase duration, and causes viability defects, none of which are due to activation of the S phase checkpoint. Using genome-wide replication assays, I found th |
| 520 | |
▼a The viability defects induced by the depletion of these two limiting initiation factors suggested that the genome is destabilized by the systemic reduction of replication efficiency. Using pulsed-field gel electrophoresis to assay the integrity |
| 520 | |
▼a In summary, the cellular abundance of Sld2 and Sld3 contributes mostly to origin firing efficiency. In contrast to a recently proposed idea that origin firing time is a secondary consequence of origin efficiency, my work detailed in this dissert |
| 590 | |
▼a School code: 0250. |
| 650 | 4 |
▼a Molecular biology. |
| 650 | 4 |
▼a Genetics. |
| 650 | 4 |
▼a Biology. |
| 690 | |
▼a 0307 |
| 690 | |
▼a 0369 |
| 690 | |
▼a 0306 |
| 710 | 20 |
▼a University of Washington.
▼b Molecular and Cellular Biology. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-12B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0250 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T14998175
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자 |