| LDR | | 03128nmm uu200493 4500 |
| 001 | | 000000331972 |
| 005 | | 20240805165743 |
| 008 | | 181129s2018 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438169357 |
| 035 | |
▼a (MiAaPQ)AAI10826088 |
| 035 | |
▼a (MiAaPQ)umn:19282 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 082 | 0 |
▼a 574 |
| 100 | 1 |
▼a Xia, Shunxiang. |
| 245 | 10 |
▼a Biocatalytic Carbon Capture and Conversion. |
| 260 | |
▼a [S.l.] :
▼b University of Minnesota.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 212 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B. |
| 500 | |
▼a Adviser: Ping Wang. |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of Minnesota, 2018. |
| 520 | |
▼a As the most significant anthropogenic greenhouse gas, carbon dioxide emitted as an industrial pollution waste can be probably most effectively handled by the Carbon Capture and Storage (CCS) strategy. The energy efficiency of currently available |
| 520 | |
▼a Among the numerous CO2-conversion and CO2-elution reaction pathways found in biology, the isocitrate dehydrogenase (ICDH) reaction in reductive tricarboxylic acid (RTCA) cycle was taken as our study model system due to its reversibility and agai |
| 520 | |
▼a To enhance reaction kinetics, screening ideal enzyme and optimizing reaction condition were conducted. ICDH from Chlorobium limicola revealed the highest specific activity: 15.3 U/mg at pH 6 for carboxylation and 90.2 U/mg at pH 9 for decarboxyl |
| 520 | |
▼a For carbon capture capacity, additional thermodynamic driving force can be created via designing of cascade reaction scheme. In this study, aconitase was introduced and isocitrate was further converted to citrate, lowing the overall Gibbs free e |
| 520 | |
▼a Enzyme immobilization was conducted to improve system stability. During the process, 20~30 mg enzyme can be absorbed on 1 gram of mesoporous silicon foam (MSF) within 5 mins with the specific activity of decarboxylation in the order of 6 U per g |
| 520 | |
▼a With economic analysis, the ICDH strategy can bring a 66% improvement in energy efficiency. The cost and stability of cofactor (NADPH) were the most significant barriers to the large-scale application of ICDH, mainly due to the poor stability of |
| 520 | |
▼a This work demonstrates the feasibility of a novel carbon capture strategy applying reversible enzyme reactions. It reveals that reaction equilibrium can be effectively by manipulating pH and reaction pathways. The reaction intensity and stabilit |
| 590 | |
▼a School code: 0130. |
| 650 | 4 |
▼a Biochemistry. |
| 650 | 4 |
▼a Energy. |
| 650 | 4 |
▼a Chemical engineering. |
| 690 | |
▼a 0487 |
| 690 | |
▼a 0791 |
| 690 | |
▼a 0542 |
| 710 | 20 |
▼a University of Minnesota.
▼b Biosystems and Agricultural Engineering. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-12B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0130 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T14998844
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자 |