| LDR | | 02687nmm uu200469 4500 |
| 001 | | 000000331922 |
| 005 | | 20240805165640 |
| 008 | | 181129s2018 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438250239 |
| 035 | |
▼a (MiAaPQ)AAI10826502 |
| 035 | |
▼a (MiAaPQ)ucsf:11624 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 082 | 0 |
▼a 574 |
| 100 | 1 |
▼a Samocha, Alexandr Joseph. |
| 245 | 10 |
▼a Mammary Epithelial Cell Fate & EGFR-Rasgrpl-Ras Effector Signals. |
| 260 | |
▼a [S.l.] :
▼b University of California, San Francisco.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 209 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B. |
| 500 | |
▼a Advisers: Jeroen P. Roose |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of California, San Francisco, 2018. |
| 520 | |
▼a Mammary stem cells (MaSC) are a heterogeneous population that give rise to progenitor cells subsequently developing into differentiated cells. The exact mammary epithelial cell (MEC) hierarchy and particularly the signals that govern this hierar |
| 520 | |
▼a We demonstrate that RasGEF Rasgrp1 is expressed in mammary epithelium and use cleared fat pad approaches to reveal a previously unrecognized functional role for RasGRP1 in the mammary gland. These approaches involved the transplantation of mamma |
| 520 | |
▼a Functionally, Rasgrp1 perturbation led to shortened mammary ductal trees in mammary whole mounts, increases in numbers of proliferative terminal end buds (TEBs), and sustained proliferation of mammary ductal cells (with high phospho-Akt) in area |
| 520 | |
▼a MEC colony assays using Rasgrp1-/- and Rasgrp1Anaef cells revealed that progenitors form more and larger colonies than WT counterparts and with increased proliferation (Ki67) in response to EGF and strong staining for pAkt, all of which were re |
| 520 | |
▼a In sum, we provide mechanistic insights on the biochemical signals that impact MACSs and progenitor cells. We show that Rasgrp1 is required to balance MEC EGFR signals and regulate mammary stem and progenitor cell fate to dampen proliferation so |
| 590 | |
▼a School code: 0034. |
| 650 | 4 |
▼a Developmental biology. |
| 650 | 4 |
▼a Cellular biology. |
| 650 | 4 |
▼a Molecular biology. |
| 690 | |
▼a 0758 |
| 690 | |
▼a 0379 |
| 690 | |
▼a 0307 |
| 710 | 20 |
▼a University of California, San Francisco.
▼b Biomedical Sciences. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-12B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0034 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T14998896
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자 |