| LDR | | 02568nmm uu200457 4500 |
| 001 | | 000000331438 |
| 005 | | 20240805164401 |
| 008 | | 181129s2018 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438194779 |
| 035 | |
▼a (MiAaPQ)AAI10927907 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 082 | 0 |
▼a 574 |
| 100 | 1 |
▼a Sherlock, Madeline Elizabeth. |
| 245 | 10 |
▼a Revealing Hidden Biology by Identifying Ligands for the ykkC Orphan Riboswitches. |
| 260 | |
▼a [S.l.] :
▼b Yale University.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 299 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B. |
| 500 | |
▼a Adviser: Ronald R. Breaker. |
| 502 | 1 |
▼a Thesis (Ph.D.)--Yale University, 2018. |
| 520 | |
▼a Riboswitches are non-coding RNAs that bind a small molecule or ion ligand to regulate gene expression. The cognate ligands have been identified for dozens of riboswitch classes, but the ligand identities are unknown for many promising structured |
| 520 | |
▼a My colleagues found that guanidine induces expression of a riboswitch- lacZ reporter gene fusion in a large screen of various growth conditions. They collected additional evidence implicating guanidine as the elusive ligand for ykkC riboswitches |
| 520 | |
▼a I subsequently determined that representatives of two other RNA motifs, called mini-ykkC and ykkC-III, also bind guanidine in vitro. All three guanidine-binding RNA motifs have similar downstream gene associations, and therefore these three dist |
| 520 | |
▼a While the majority of ykkC RNAs selectively bind guanidine, I found that the remaining 30% of ykkC RNAs do not bind guanidine and carry nucleotide changes in their binding pockets to allow recognition of a different ligand. I analyzed the consen |
| 520 | |
▼a I discovered that the first variant class selectively binds guanosine tetraphosphate (ppGpp), a bacterial alarmone derived from the ribonucleotide GTP. The ppGpp riboswitch class primarily regulates transcription of genes involved in branched-ch |
| 590 | |
▼a School code: 0265. |
| 650 | 4 |
▼a Biochemistry. |
| 650 | 4 |
▼a Microbiology. |
| 650 | 4 |
▼a Molecular biology. |
| 690 | |
▼a 0487 |
| 690 | |
▼a 0410 |
| 690 | |
▼a 0307 |
| 710 | 20 |
▼a Yale University. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-12B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0265 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15000857
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자 |