| LDR | | 00000nmm u2200205 4500 |
| 001 | | 000000330856 |
| 005 | | 20241105133917 |
| 008 | | 181129s2018 ||| | | | eng d |
| 020 | |
▼a 9780438126473 |
| 035 | |
▼a (MiAaPQ)AAI10903041 |
| 035 | |
▼a (MiAaPQ)umichrackham:001218 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 049 | 1 |
▼f DP |
| 082 | 0 |
▼a 621 |
| 100 | 1 |
▼a Ho, Kenneth Kwun Yin. |
| 245 | 10 |
▼a Progressing Mechanobiology from a Simplified to a More Complex System: Development of Novel Platforms and Investigation of Actin Cytoskeletal Remodeling. |
| 260 | |
▼a [S.l.] :
▼b University of Michigan.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 149 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B. |
| 500 | |
▼a Adviser: Allen Po-Chih Liu. |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of Michigan, 2018. |
| 520 | |
▼a Compression is a common mechanical stimulus in our body that results from gravity and tissue growth. Inability for cells to maintain force homeostasis can lead to various diseases, and cells may experience an increase in compressive stress in di |
| 520 | |
▼a First, I developed an approach that combines purified actin cytoskeletal proteins or mammalian cell-free expression and ultra-thin double emulsion template for constructing a simplified model of a cell through in vitro reconstitution. This was u |
| 520 | |
▼a I also developed a pneumatic-controlled, two-layered microfluidic platform for applying compression and aspiration to double emulsion droplets as a model cell. I further improved the microfluidic device to apply controlled compression to single |
| 520 | |
▼a Finally, I investigated actin cytoskeletal remodeling, mechanosensing and mechanotransduction of epithelial cells under compression, in a 2D cell population context. I discovered the formation of actin protrusions at the apical surface of the ce |
| 520 | |
▼a In this dissertation, I developed experimental platforms and studied the remodeling of actin networks under compression using in vitro reconstitution, single-cell and cell population systems, taking advantages of each experimental system. The p |
| 590 | |
▼a School code: 0127. |
| 650 | 4 |
▼a Mechanical engineering. |
| 690 | |
▼a 0548 |
| 710 | 20 |
▼a University of Michigan.
▼b Mechanical Engineering. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-12B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0127 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15000541
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자
▼b 관리자 |