| LDR | | 00000nmm u2200205 4500 |
| 001 | | 000000330854 |
| 005 | | 20241105133659 |
| 008 | | 181129s2018 ||| | | | eng d |
| 020 | |
▼a 9780438126459 |
| 035 | |
▼a (MiAaPQ)AAI10903039 |
| 035 | |
▼a (MiAaPQ)umichrackham:001140 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 049 | 1 |
▼f DP |
| 082 | 0 |
▼a 621 |
| 100 | 1 |
▼a Heureaux-Torres, Johanna L. |
| 245 | 10 |
▼a Bacterial Mechanosensitive Channel of Large Conductance (MscL) in Mammalian Cells for Novel Mechanobiology Applications. |
| 260 | |
▼a [S.l.] :
▼b University of Michigan.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 99 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B. |
| 500 | |
▼a Adviser: Allen Po-Chih Liu. |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of Michigan, 2018. |
| 520 | |
▼a Mechanobiology, a relatively young field that centers on how external physical forces on cells or tissues and their intrinsic mechanical properties can influence physiology and disease, has become a pillar in cell biology. Indeed, cells experien |
| 520 | |
▼a Mechanotransduction at the cellular-scale has perceptible, large-scale implications such as proper organism development, our ability to sense sound and touch, function and homeostasis of organ systems, and disease progression. Previous work in m |
| 520 | |
▼a Here we demonstrate how the functional expression of the E. coli membrane tension gated mechanosensitive channel of large conductance (MscL) in mammalian cells endows the cells with new mechano-sensing capabilities such as the activation of Msc |
| 520 | |
▼a The first major contribution in this thesis was to show that MscL can be expressed in mammalian cells, localize to cellular membranes, and responds to membrane tension via osmotic down-shock. The second contribution was demonstrating that the ac |
| 520 | |
▼a Lastly, we investigated the effect of MscL mechanotransduction on the cell function of migration in cancer metastasis and then more specifically, 3D-confinements. Our findings in our in vivo mouse model showed that there was a marked reduction i |
| 520 | |
▼a Implications of this thesis are that MscL: (1) can be used as a molecular delivery tool for live-cells via mechanical stimulus |
| 590 | |
▼a School code: 0127. |
| 650 | 4 |
▼a Mechanical engineering. |
| 690 | |
▼a 0548 |
| 710 | 20 |
▼a University of Michigan.
▼b Mechanical Engineering. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-12B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0127 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15000539
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자
▼b 관리자 |