| LDR | | 02511nmm uu200421 4500 |
| 001 | | 000000330842 |
| 005 | | 20240805162927 |
| 008 | | 181129s2018 |||||||||||||||||c||eng d |
| 020 | |
▼a 9780438126268 |
| 035 | |
▼a (MiAaPQ)AAI10903020 |
| 035 | |
▼a (MiAaPQ)umichrackham:001199 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 082 | 0 |
▼a 616 |
| 100 | 1 |
▼a Malec, Paige A. |
| 245 | 10 |
▼a Derivatization Methods for Improved Metabolome Analysis by LC-MS/MS. |
| 260 | |
▼a [S.l.] :
▼b University of Michigan.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 163 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B. |
| 500 | |
▼a Adviser: Robert T. Kennedy. |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of Michigan, 2018. |
| 520 | |
▼a Metabolomics, the study and characterization of small molecule metabolites, faces challenges due to the size and diversity of the metabolome. No current instrumentation is capable of measuring the entire metabolome. Liquid chromatography with ma |
| 520 | |
▼a One way to overcome this limitation is through the use of chemical derivatization. Polar metabolites are labeled with a hydrophobic moiety, increasing retention and ionization efficiency in reversed phase chromatography, allowing for lower limit |
| 520 | |
▼a In this work, benzoyl chloride derivatization is expanded upon in both metabolome coverage and applicability. To allow benzoyl chloride derivatization of proteinaceous samples such as plasma or tissue, a sample preparation method was developed a |
| 520 | |
▼a With sample preparation optimized, we then aimed to expand metabolome coverage and subsequently developed a method for the analysis of 70 benzoylated neurochemicals. With nanomolar limits of detection and internal standards for each metabolite, |
| 520 | |
▼a Finally, we move away from benzoyl chloride and introduce a novel derivatization reagent, benzylamine. Benzylamine derivatization is complementary to benzoyl chloride, reacting with carboxylic acids, carbonyls, and phosphates. Moreover, the two |
| 590 | |
▼a School code: 0127. |
| 650 | 4 |
▼a Neurosciences. |
| 690 | |
▼a 0317 |
| 710 | 20 |
▼a University of Michigan.
▼b Chemistry. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-12B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0127 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T15000527
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자 |