| LDR | | 00000nmm u2200205 4500 |
| 001 | | 000000330399 |
| 005 | | 20241029160921 |
| 008 | | 181129s2018 ||| | | | eng d |
| 020 | |
▼a 9780438383920 |
| 035 | |
▼a (MiAaPQ)AAI10844477 |
| 035 | |
▼a (MiAaPQ)colorado:15632 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 049 | 1 |
▼f DP |
| 082 | 0 |
▼a 540 |
| 100 | 1 |
▼a Grau, William Clifford. |
| 245 | 10 |
▼a Application of Advances in Synthetic Biology to Modular Megasynthase Design and Scalable Combinatorial Biosynthesis. |
| 260 | |
▼a [S.l.] :
▼b University of Colorado at Boulder.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 188 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 80-02(E), Section: B. |
| 500 | |
▼a Adviser: Ryan T. Gill. |
| 502 | 1 |
▼a Thesis (Ph.D.)--University of Colorado at Boulder, 2018. |
| 520 | |
▼a The diversity in structure and biological activity of natural products has long captured the imagination of chemists and molecular biologists alike. Natural products are synthesized by elaborate secondary metabolic pathways or enzymes. For examp |
| 520 | |
▼a Prior efforts to rationally design modular megasynthases have demonstrated some success but combinatorial forward engineering remains unfulfilled. The rise of synthetic biology has presented new capabilities to resolve the underlying deficiencie |
| 520 | |
▼a Herein, we present a precise and scalable synthetic biology approach to combinatorial biosynthesis built on improved abstraction of modular megasynthases into 'parts'. The approach begins with bottom-up design of a chimeric modular megasynthase |
| 520 | |
▼a To demonstrate this approach, we first targeted the production of a triketide lactone food and fragrance ingredient, d-hexalactone. A retrosynthetic analysis identified catalytic domains, linkers, and substrates required for d-hexalactone biosyn |
| 590 | |
▼a School code: 0051. |
| 650 | 4 |
▼a Chemistry. |
| 650 | 4 |
▼a Bioengineering. |
| 650 | 4 |
▼a Chemical engineering. |
| 690 | |
▼a 0485 |
| 690 | |
▼a 0202 |
| 690 | |
▼a 0542 |
| 710 | 20 |
▼a University of Colorado at Boulder.
▼b Chemistry. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 80-02B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0051 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T14999991
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자
▼b 관리자 |