| LDR | | 00000nmm u2200205 4500 |
| 001 | | 000000330024 |
| 005 | | 20241017163231 |
| 008 | | 181129s2018 ||| | | | eng d |
| 020 | |
▼a 9780438206229 |
| 035 | |
▼a (MiAaPQ)AAI10683071 |
| 035 | |
▼a (MiAaPQ)rpi:11217 |
| 040 | |
▼a MiAaPQ
▼c MiAaPQ
▼d 248032 |
| 049 | 1 |
▼f DP |
| 082 | 0 |
▼a 660 |
| 100 | 1 |
▼a Pearson, C. Seth. |
| 245 | 10 |
▼a Inteins as Biomolecular Switches: Applications in Drug Discovery, Protein Purification, and Stress Response. |
| 260 | |
▼a [S.l.] :
▼b Rensselaer Polytechnic Institute.,
▼c 2018 |
| 260 | 1 |
▼a Ann Arbor :
▼b ProQuest Dissertations & Theses,
▼c 2018 |
| 300 | |
▼a 102 p. |
| 500 | |
▼a Source: Dissertation Abstracts International, Volume: 79-12(E), Section: B. |
| 500 | |
▼a Advisers: Georges Belfort |
| 502 | 1 |
▼a Thesis (Ph.D.)--Rensselaer Polytechnic Institute, 2018. |
| 520 | |
▼a Inteins are protein elements that autocatalytically excise themselves from a host protein in a process called protein splicing. The two flanking sequences of the host protein, called the N- and C-exteins, are subsequently ligated with a native p |
| 520 | |
▼a During this process, the intein breaks two peptide bonds and forms a third. Taking advantage of the intein's ability to catalyze these reactions, both within the natural host as well as in vitro for biotechnological applications, provides an att |
| 520 | |
▼a Our work focuses on two of the three inteins found in the human pathogen Mycobacterium tuberculosis (Mtu), RecA and SufB. The intein-interrupted proteins are important for the viability of Mtu. Preventing intein excision, and thus the formation |
| 520 | |
▼a Turning to methods of control for in vitro applications, we introduced a mutation in Mtu RecA that causes the intein to spontaneously form a thiazoline ring at the N-terminal splicing junction. While present, the thiazoline ring prevents any fur |
| 520 | |
▼a The final aspect of our work is based on the intriguing hypothesis that inteins are not simply parasitic elements, but offer a beneficial protein-level control of host protein function. We demonstrated that Mtu SufB activity is inhibited in vivo |
| 520 | |
▼a Overall, this work investigates strategies for controlling inteins in the realms of drug development, protein purification, and host regulation and stress response. |
| 590 | |
▼a School code: 0185. |
| 650 | 4 |
▼a Chemical engineering. |
| 650 | 4 |
▼a Biochemistry. |
| 690 | |
▼a 0542 |
| 690 | |
▼a 0487 |
| 710 | 20 |
▼a Rensselaer Polytechnic Institute.
▼b Chemical Engineering. |
| 773 | 0 |
▼t Dissertation Abstracts International
▼g 79-12B(E). |
| 773 | |
▼t Dissertation Abstract International |
| 790 | |
▼a 0185 |
| 791 | |
▼a Ph.D. |
| 792 | |
▼a 2018 |
| 793 | |
▼a English |
| 856 | 40 |
▼u http://www.riss.kr/pdu/ddodLink.do?id=T14996731
▼n KERIS |
| 980 | |
▼a 201812
▼f 2019 |
| 990 | |
▼a 관리자
▼b 관리자 |