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019 ▼a 1201227716
020 ▼a 0262353970
020 ▼a 9780262353977 ▼q (electronic bk.)
020 ▼z 9780262042659
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050 4 ▼a R857.B54
08204 ▼a 610.284
1001 ▼a Wittrup, K. Dane.
24510 ▼a Quantitative Fundamentals of Molecular and Cellular Bioengineering ▼h [electronic resource].
260 ▼a Cambridge : ▼b MIT Press, ▼c 2020.
300 ▼a 1 online resource (568 p.)
500 ▼a Description based upon print version of record.
500 ▼a Case Study 3-1 J. D. Stone, J. R. Cochran, and L. J. Stern. T-cell activation by soluble MHC oligomers can be described by a two-parameter binding model. Biophys. J. 81: 2547-2557 (2001).
5050 ▼a Intro -- Title Page -- Copyright -- Table of Contents -- Dedication -- Preface and Acknowledgments -- 1. Introduction to Biological Rate Processes -- 1.1. Biological Rate Processes -- 1.2. Why Develop a Model? -- 1.3. Mechanistic Model Formulation -- 1.4. Model Validation -- 1.4.1. Consistency with Experiment Is Necessary but Not Sufficient for a Model's Correctness -- 1.4.2. Comparison to Alternative Models -- 1.4.3. Prediction and Falsifiability -- 1.4.4. Parameter Sensitivity Analysis -- 1.5. Basic Themes in Rate Process Modeling -- 1.5.1. Steady State versus Equilibrium
5058 ▼a 1.5.2. Perturbations -- 1.5.3. Rate Forms -- 1.5.4. Characteristic Time and Length Scales -- Suggestions for Further Reading -- References -- 2. Noncovalent Binding Interactions -- 2.1. Kinetic Rate Constants -- 2.1.1. Typical Ranges for kon and koff -- 2.2. Thermodynamics -- 2.2.1. State Functions -- 2.2.2. Free Energy and Standard States -- 2.3. Energetic Contributions to Binding Affinity -- 2.3.1. Electrostatics -- 2.3.2. Hydrogen Bonding -- 2.3.3. van der Waals Interactions -- 2.3.4. Hydrophobic Effect -- 2.3.5. Deformation Energy and Entropy -- 2.4. Energetics of Protein Binding Interfaces
5058 ▼a 2.4.1. Thermodynamic Cycle Analysis -- Case Study 2-1 P. J. Carter, G. Winter, A. J. Wilkinson, and A. R. Fersht. The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus). Cell 38: 835-840 (1984). -- Case Study 2-2 Z. S. Hendsch and B. Tidor. Do salt bridges stabilize proteins? A continuum electrostatic analysis. Protein Sci. 3: 211-226 (1994). -- 2.5. Environmental Impacts on Binding Rate -- 2.5.1. Arrhenius Relationship and Transition State Theory -- 2.5.2. Electrostatic Effects on Binding Kinetics
5058 ▼a 2.6. Molecular Measurements: Light-Matter Interactions -- 2.6.1. Scattering -- 2.6.2. Absorbance -- 2.6.3. Fluorescence -- Case Study 2-3 L. Song, E. J. Hennink, I. T. Young, and H. J. Tanke. Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy. Biophys. J. 68: 2588-2600 (1995). -- 2.7. Fluorescence Applications for Biomolecular Measurements -- 2.7.1. Biosynthetic Fluorescent Labeling -- Case Study 2-4 B. G. Reid and G. C. Flynn. Chromophore formation in green fluorescent protein. Biochemistry 36: 6786-6791 (1997). -- 2.7.2. Common Fluorophores for In Vitro Conjugation
5058 ▼a 2.7.3. Fluorescence Instrumentation -- Suggestions for Further Reading -- Problems -- References -- 3. Binding Equilibria and Kinetics -- 3.1. Equilibrium Monovalent Protein-Ligand Binding -- 3.1.1. Monovalent Binding Isotherm -- 3.1.2. Graphical Representations -- 3.2. Binding Kinetics -- 3.3. Multiple Binding Sites -- 3.3.1. Independent Sites -- 3.3.2. Cooperativity -- 3.3.3. Avidity and Effective Concentration -- 3.3.4. Equilibrium Bivalent Binding at Cell Surfaces
520 ▼a A comprehensive presentation of essential topics for biological engineers, focusing on the development and application of dynamic models of biomolecular and cellular phenomena.
650 0 ▼a Science.
650 7 ▼a SCIENCE / Life Sciences / Molecular Biology. ▼2 bisacsh
650 7 ▼a Science. ▼2 fast ▼0 (OCoLC)fst01108176
655 4 ▼a Electronic books.
7001 ▼a Tidor, Bruce.
7001 ▼a Hackel, Benjamin J.
7001 ▼a Sarkar, Casim A.
77608 ▼i Print version: ▼a Wittrup, K. Dane ▼t Quantitative Fundamentals of Molecular and Cellular Bioengineering ▼d Cambridge : MIT Press,c2020 ▼z 9780262042659
85640 ▼3 EBSCOhost ▼u https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=2654327
938 ▼a Askews and Holts Library Services ▼b ASKH ▼n AH37793732
938 ▼a Recorded Books, LLC ▼b RECE ▼n rbeEB00838329
938 ▼a ProQuest Ebook Central ▼b EBLB ▼n EBL6374134
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938 ▼a EBSCOhost ▼b EBSC ▼n 2654327
990 ▼a 관리자