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▼2 bisacsh |
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▼a 543.65
▼a 572.4 |
| 100 | 1 |
▼a Lutz, Norbert. |
| 245 | 10 |
▼a Methodologies for Metabolomics :
▼b Experimental Strategies and Techniques.
▼h [electronic resource] |
| 260 | |
▼a Cambridge :
▼b Cambridge University Press,
▼c 2012. |
| 300 | |
▼a 1 online resource (642 pages) |
| 336 | |
▼a text
▼b txt
▼2 rdacontent |
| 337 | |
▼a computer
▼b c
▼2 rdamedia |
| 338 | |
▼a online resource
▼b cr
▼2 rdacarrier |
| 500 | |
▼a 2. Considerations in the Growth, Harvest, Preparation, and Extraction of Plant Materials. |
| 504 | |
▼a Includes bibliographical references and index. |
| 505 | 0 |
▼a Contributors; Section 1 Basic Methodological Strategies in Metabolomic Research; 1 Exploring the Human Metabolome by Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry; 1. Brief History of Human Metabolomics; 2. Sample Preparation for Human Metabolomic Studies; 3. Human Metabolite Identification by Nuclear Magnetic Resonance Spectroscopy; 4. Human Metabolite Identification by Gas Chromatography-Mass Spectrometry; 5. Human Metabolite Identification by Liquid Chromatography-Mass Spectrometry; 6. Comparison of Technologies; 7. Conclusion; Acknowledgments. |
| 505 | 8 |
▼a 2 Methodological Requirements for Lipidomics Research1. Sample Preparation and Lipid Extraction; 2. Sample Preparation; 3. Lipid Extraction; 4. Sample Preparation and Lipid Extraction of Subcellular Organelles; 5. Chromatography-Coupled Mass Spectrometry of Lipids; 6. Shotgun Lipidomics -- Direct Infusion-Based Mass Spectrometry of Lipids; 7. Postacquisition Data Processing and Bioinformatics; 8. Conclusion; Acknowledgments; 3 Biological Methods for Metabolic Research; 1. Gene Overexpression as a Tool for Metabolomic Studies; 1.1. Gene Transfection: Plasmid Vectors. |
| 505 | 8 |
▼a 1.2. Gene Transduction: Viral Vectors2. Gene Silencing as a Tool for Metabolomic Studies; 3. Validation of Gene Overexpression or Silencing by mRNA; 3.1. Gene Expression Analysis; 3.1.1. Reverse Transcriptase Polymerase Chain Reaction; 3.1.2. Real-Time Polymerase Chain Reaction; 4. Validation of Gene Overexpression or Silencing by Protein Level Analysis and Enzyme Assays; 4.1. Western Blotting; 4.1.1. Blotting; 4.1.2. Immunodetection; 4.2. Enzyme Assays; 4.2.1. Protocols for Analysis of Choline Kinase- Activity; 4.2.2. Protocol for Analysis of Phospholipase D Activity. |
| 505 | 8 |
▼a 5. Enzymatic Assays as a Tool of Phospholipid Metabolism Analysis: Effects of Choline Kinase- Inhibition on the Balance of Phospholipid Metabolites5.1. Protocol for Lipid Analysis; 5.2. Protocol for Analysis of Phosphatidylinositol-3-Kinase Activity; 6. Phospholipid Profile Analysis by Mass Spectrometry; 6.1. Phospholipid Extraction; 6.2. Analytical Technique for Phospholipids: Mass Spectrometry; Section 2 Metabolomic Mass Spectrometry: Experimental Techniques and Bioinformatics. |
| 505 | 8 |
▼a 4 Considerations in Sample Preparation, Collection, and Extraction Approaches Applied in Microbial, Plant, and Mammalian Metabolic Profiling1. Considerations in the Culturing, Collection, Preparation, and Extraction of Microbial Samples; 1.1. Sample Types; 1.2. Culture Methods; 1.2.1. Batch Growth; 1.2.2. Continuous Culture; 1.2.3. Selection of Media; 1.3. Experimental Considerations in the Collection and Preparation of Samples; 1.3.1. Quenching of Cultures; 1.3.2. Assessment of Leakage; 1.3.3. Extraction of Intracellular Metabolites. |
| 520 | |
▼a Methodologies for Metabolomics provides comprehensive descriptions of the newest methodological strategies and techniques in metabolomic research. |
| 546 | |
▼a English. |
| 588 | 0 |
▼a Print version record. |
| 590 | |
▼a Master record variable field(s) change: 650 |
| 650 | 0 |
▼a Mass spectrometry
▼x Methods. |
| 650 | 0 |
▼a Metabolism
▼x Disorders
▼x Diagnosis. |
| 650 | 0 |
▼a Metabolites. |
| 650 | 2 |
▼a Metabolomics
▼x methods. |
| 650 | 2 |
▼a Metabolome. |
| 650 | 2 |
▼a Chemistry Techniques, Analytical. |
| 650 | 2 |
▼a Biochemistry. |
| 650 | 2 |
▼a Computational Biology. |
| 650 | 2 |
▼a Analytical, Diagnostic and Therapeutic Techniques and Equipment. |
| 650 | 2 |
▼a Metabolism. |
| 650 | 2 |
▼a Biology. |
| 650 | 2 |
▼a Disease. |
| 650 | 2 |
▼a Phenomena and Processes. |
| 650 | 2 |
▼a Biological Science Disciplines. |
| 650 | 2 |
▼a Chemistry. |
| 650 | 2 |
▼a Natural Science Disciplines. |
| 650 | 2 |
▼a Disciplines and Occupations. |
| 650 | 2 |
▼a Diagnosis. |
| 650 | 2 |
▼a Mass Spectrometry. |
| 650 | 2 |
▼a Metabolic Diseases. |
| 650 | 7 |
▼a SCIENCE
▼x Chemistry
▼x Analytic.
▼2 bisacsh |
| 655 | 4 |
▼a Electronic books. |
| 700 | 1 |
▼a Sweedler, Jonathan. |
| 700 | 1 |
▼a Wevers, Ron. |
| 776 | 08 |
▼i Print version:
▼a Lutz, Norbert.
▼t Methodologies for Metabolomics : Experimental Strategies and Techniques.
▼d Cambridge : Cambridge University Press, 짤2012
▼z 9780521765909 |
| 856 | 40 |
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